Journal
NATURE BIOMEDICAL ENGINEERING
Volume 4, Issue 3, Pages 286-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41551-020-0527-8
Keywords
-
Categories
Funding
- NCI NIH HHS [R01 CA212379, P30 CA008748, R01 CA183953] Funding Source: Medline
Ask authors/readers for more resources
High-resolution raster-scanning optoacoustic mesoscopy provides detailed morphological and physiological insights into the responses of tumours to vascular-targeted therapies. The monitoring of vascular-targeted therapies using magnetic resonance imaging, computed tomography or ultrasound is limited by their insufficient spatial resolution. Here, by taking advantage of the intrinsic optical properties of haemoglobin, we show that raster-scanning optoacoustic mesoscopy (RSOM) provides high-resolution images of the tumour vasculature and of the surrounding tissue, and that the detection of a wide range of ultrasound bandwidths enables the distinction of vessels of differing size, providing detailed insights into the vascular responses to vascular-targeted therapy. Using RSOM to examine the responses to vascular-targeted photodynamic therapy in mice with subcutaneous xenografts, we observed a substantial and immediate occlusion of the tumour vessels followed by haemorrhage within the tissue and the eventual collapse of the entire vasculature. Using dual-wavelength RSOM, which distinguishes oxyhaemoglobin from deoxyhaemoglobin, we observed an increase in oxygenation of the entire tumour volume immediately after the application of the therapy, and a second wave of oxygen reperfusion approximately 24 h thereafter. We also show that RSOM enables the quantification of differences in neoangiogenesis that predict treatment efficacy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available