Impact of Growth Stage and Biomass Fractions on Cannabinoid Content and Yield of Different Hemp (Cannabis sativa L.) Genotypes

The medicinal use of cannabinoids renewed the interest in industrial hemp (Cannabis sativa L.). The aim of this study was to evaluate the impact of growth stage and biomass fractions of seven industrial hemp genotypes. The study focused on biomass yield, content of cannabidiolic acid/cannabidiol (CBDA/CBD), cannabigerolic acid/cannabigerol (CBGA/CBG), and tetrahydrocannabinolic acid (THCA). The experiment was conducted in 2017 and 2018. The biomass samples were taken at the vegetative (S1), bud (S2), full-flowering (S3) and seed maturity stage (S4). Plants were fractionated into inflorescence, upper and lower leaves. The average inflorescence dry yield of genotypes Futura75, Fedora17, Felina32 and Ferimon ranged between 257.28 g m(-2) to 442.00 g m(-2), resulting in a maximum yield of CBDA at S4, with 4568.26 mg m(-2), 6011.20 mg m(-2), 4975.60 mg m(-2) and 1929.60 mg m(-2), respectively. CBGA was exclusively found in genotype Santhica27, with a maximum CBGA yield of 5721.77 mg m(-2) in inflorescence at growth stage S4 and a dry weight yield of 408.99 g m(-2). Although these industrial hemp genotypes are mainly cultivated for fibre and seed production, however, cannabinoids offer an additional value. For an optimized harvest result, yield of extractable material and overall yield of cannabinoids must be considered.