Journal
ANIMALS
Volume 10, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/ani10060944
Keywords
sheep; intersex; whole-genome resequencing; copy number variation; forming mechanism
Funding
- National Natural Science Foundation of China [31660642, 31360529, 31760649]
- Fundamentai Research Funds for the Central Universities [31920190020, 31920190004]
- Science-technology Support Plan Project of Gansu Province [18JR3RA373,18YF1FA121]
- Program for Changjiang Scholars and Innovative Research Team in the University [IRT_17R88]
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Simple Summary Individuals with hermaphroditism are a serious hazard to animal husbandry and production due to their abnormal fertility. The molecular mechanism of sheep intersex formation was unclear. This study was the first to locate the homologous sequence of the goat polled intersex syndrome (PIS) region in sheep and found that the intersex traits of Lanzhou fat-tailed sheep were not caused by the lack of this region. By detecting the selective sweep regions, vital genes associated with androgen biosynthesis and the follicle stimulating hormone response entry were found, includingsteroid 5 alpha-reductase 2(SRD5A2), andpro-apoptotic WT1 regulator (PAWR). Additionally, the copy number variations of the four regions on chr9, chr1, chr4, and chr16 may affect the expression of the gonadal development genes,zinc finger protein,FOG family member 2(ZFPM2),LIM homeobox 8(LHX8),inner mitochondrial membrane peptidase subunit 2(IMMP2L) andslit guidance ligand 3(SLIT3), respectively, and further affect the formation of intersex traits. Intersex, also known as hermaphroditism, is a serious hazard to animal husbandry and production. The mechanism of ovine intersex formation is not clear. Therefore, genome-wide resequencing on the only two intersex and two normal Lanzhou fat-tailed (LFT) sheep, an excellent but endangered Chinese indigenous sheep breed, was performed. Herein, the deletion of homologous sequences of the goat polled intersex syndrome (PIS) region (8787 bp, 247747059-247755846) on chromosome 1 of the LFT sheep was not the cause of the ovine intersex trait. By detecting the selective sweep regions, we found that the genes related to androgen biosynthesis and follicle stimulating hormone response items, such assteroid 5 alpha-reductase 2(SRD5A2),steroid 5 alpha-reductase 3(SRD5A3), andpro-apoptotic WT1 regulator (PAWR), may be involved in the formation of intersex traits. Furthermore, the copy number variations of the four regions, chr9: 71660801-71662800, chr1: 50776001-50778000, chr4: 58119201-58121600, and chr16: 778801-780800, may affect the expression of thezinc finger protein,FOG family member 2(ZFPM2),LIM homeobox 8(LHX8),inner mitochondrial membrane peptidase subunit 2(IMMP2L) andslit guidance ligand 3(SLIT3) genes, respectively, which contribute to the appearance of intersex traits. These results may supply a theoretical basis for the timely detection and elimination of intersex individuals in sheep, which could accelerate the healthy development of animal husbandry.
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