Journal
SCIENCE ADVANCES
Volume 6, Issue 22, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aba6712
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Funding
- ImPACT Program of the Council for Science, Technology, and Innovation (Cabinet Office, Government of Japan)
- JSPS KAKENHI [JP18K14101]
- JSPS Core-to-Core Program
- JST Adaptable and Seamless Technology transfer Program [JPMJTM19Y9]
- Kanagawa Institute of Industrial Science and Technology (KISTEC)
- White Rock Foundation
- Murata Science Foundation
- Crossministerial Strategic Innovation Promotion Program (SIP)
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Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with similar to 20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.
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