Journal
ACS SENSORS
Volume 5, Issue 4, Pages 943-951Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b02116
Keywords
monoamine oxidase A; mitochondrial-targeted; bioimaging; near-infrared fluorescence probe; hepatic fibrosis
Funding
- National Nature Science Foundation of China [21462007, 21002015, 21662004]
- State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources [CMEMR 2015-A03, 2018-C20]
- Natural Science Foundation of Guizhou Province [[2020]1Y052]
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Monoamine oxidase A (MAO-A)is a promising diagnostic marker for cancer, depression, Parkinson's disease, and liver disease. The fluorescence detection of MAO-A in living animals is of extreme importance for the early diagnosis of related diseases. However, the development of specific and mitochondrial-targeted and near-infrared (NIR) fluorescence MAO-A probes is still inadequate. Here, we designed and synthesized four NIR fluorescence probes containing a dihydroxanthene (DH) skeleton to detect MAO-A in complex biological systems. The specificity of our representative probe DHMP2 displays a 31-fold fluorescence turn-on in vitro, and it can effectively accumulate in the mitochondria and specifically detect the endogenous MAO-A concentrations in PC-3 and SH-SYSY cell lines. Furthermore, the probe DHMP2 can be used to visualize the endogenous MAO-A activity in zebrafish and tumor-bearing mice. More importantly, it is the first time that the MAO-A activity of hepatic fibrosis tissues is detected through the probe DHMP2. The present study shows that the synthesized DHMP2 might serve as a potential tool for monitoring MAO-A activity in vivo and diagnosing related diseases.
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