Journal
OPTICA
Volume 7, Issue 4, Pages 359-366Publisher
OPTICAL SOC AMER
DOI: 10.1364/OPTICA.390186
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Funding
- Precursory Research for Embryonic Science and Technology [JPMJPR17G2]
- Japan Society for the Promotion of Science [17H04852, 17K19071]
- Grants-in-Aid for Scientific Research [17H04852, 17K19071] Funding Source: KAKEN
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Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase imaging (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morphological and biochemical information, respectively. These techniques have enabled numerous applications as they have matured over the past few decades; however, their label-free contrasts are inherently complementary and difficult to integrate due to their reliance on different light-matter interactions. Here we present a unified imaging scheme with simultaneous and in situ acquisition of quantitative phase and molecular vibrational contrasts of single cells in the QPI framework using the mid-infrared photothermal effect. The robust integration of subcellular morphological and biochemical label-free measurements may enable new analyses, especially for studying complex and fragile biological phenomena such as drug delivery, cellular disease, and stem cell development, where long-time observation of unperturbed cells is needed under low phototoxicity. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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