Journal
MOLECULAR METABOLISM
Volume 40, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.molmet.2020.101026
Keywords
JAK-STAT; beta-adrenergic signalling; Thermogenesis; Temperature maintenance
Categories
Funding
- Austrian Science Fund (FWF) [SFBF04707, SFB-F06105, SFB-F06107, I 4157-B]
- EU Transcan-2 consortium ERANET-PLL via the FWF
- DLR
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [636855]
- European Research Council (ERC) [636855] Funding Source: European Research Council (ERC)
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Objective: Increasing energy expenditure through activation of brown adipose tissue (BAT) thermogenesis is an attractive approach to counteract obesity. It is therefore essential to understand the molecular mechanisms that control BAT functions. Until now several members of the Janus kinase (JAK) - signal transducer and activator of transcription (STAT) pathway have been implicated as being relevant for BAT physiology. However, whether the STAT family member STAT5 is important for the thermogenic property of adipose tissues is unknown. Therefore, we have investigated the role of STAT5 in thermogenic fat in this paper. Methods: We performed metabolic and molecular analyses using mice that harbor an adipocyte-specific deletion of Stat5a/b alleles. Results: We found that STAT5 is necessary for acute cold-induced temperature maintenance and the induction of lipid mobilization in BAT following beta(3)-adrenergic stimulation. Moreover, mitochondrial respiration of primary differentiated brown adipocytes lacking STAT5 was diminished. Increased sensitivity to cold stress upon STAT5 deficiency was associated with reduced expression of thermogenic markers including uncoupling protein 1 (UCP1), while decreased stimulated lipolysis was linked to decreased protein kinase A (PKA) activity. Additionally, brown remodeling of white adipose tissue was diminished following chronic beta(3)-adrenergic stimulation, which was accompanied by a decrease in mitochondrial performance. Conclusion: We conclude that STAT5 is essential for the functionality and the beta-adrenergic responsiveness of thermogenic adipose tissue. (C) 2020 The Author(s). Published by Elsevier GmbH.
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