Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 8, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/ab8571
Keywords
refractive index; fluorescence lifetime; FLIM; proteins condensation; membraneless organelles; liquid liquid phase separation; cellular compartmentalization
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Funding
- National Institute of General Medical Sciences of the National Institutes of Health [R44GM116193]
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Intracellular refractive index (RI) is an essential biophysical parameter, which best represents the mass and the distribution of proteins in the cell interior, including high-density accumulations in membraneless organelles. For RI measurements, a number of sophisticated techniques have been developed; however most of the new approaches are either insufficiently sensitive to intracellular variations of proteins distribution or are not compatible with live cell studies. Here, we outline the fluorescence lifetime imaging (FLIM) strategy for high resolution mapping of subcellular RI. We provide an example of our recent studies in which we utilize FLIM for measurements and monitoring of local RI in the major membraneless organelles within live cultured cells.
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