Journal
MICROBIOLOGYOPEN
Volume 9, Issue 7, Pages -Publisher
WILEY
DOI: 10.1002/mbo3.1051
Keywords
biodiesel; biosynthesis; coculture; FAEE; metabolic engineering; Y; lipolytica
Categories
Funding
- Startup Fund for Haihe Young Scholars of Tianjin University of Science and Technology
- Innovative Research Team of Tianjin Municipal Education Commission [TD13-5013]
- Research Foundation of Tianjin Municipal Education Commission, China [2017ZD03]
- Open Fund of Ministry of Education Key Laboratory of Molecular Microbiology and Technology, Nankai University
- Public Service Platform Project for Selection and Fermentation Technology of Industrial Microorganisms, China [17PTGCCX00190]
- Thousand Young Talents Program of Tianjin, China
- Natural Science Foundation of Tianjin [17JCYBJC40800]
- Ministry of Education
- Tianjin University
- Nankai University
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Fatty acid ethyl esters (FAEEs) are fatty acid-derived molecules and serve as an important form of biodiesel. The oleaginous yeast Yarrowia lipolytica is considered an ideal host platform for the production of fatty acid-derived products due to its excellent lipid accumulation capacity. In this proof-of-principle study, several metabolic engineering strategies were applied for the overproduction of FAEE biodiesel in Y. lipolytica. Here, chromosome-based co-overexpression of two heterologous genes, namely, PDC1 (encoding pyruvate decarboxylase) and ADH1 (encoding alcohol dehydrogenase) from Saccharomyces cerevisiae, and the endogenous GAPDH (encoding glyceraldehyde-3-phosphate dehydrogenase) gene of Y. lipolytica resulted in successful biosynthesis of ethanol at 70.8 mg/L in Y. lipolytica. The engineered Y. lipolytica strain expressing the ethanol synthetic pathway together with a heterologous wax ester synthase (MhWS) exhibited the highest FAEE titer of 360.8 mg/L, which is 3.8-fold higher than that of the control strain when 2% exogenous ethanol was added to the culture medium of Y. lipolytica. Furthermore, a synthetic microbial consortium comprising an engineered Y. lipolytica strain that heterologously expressed MhWS and a S. cerevisiae strain that could provide ethanol as a substrate for the production of the final product in the final engineered Y. lipolytica strain was created in this study. Finally, this synthetic consortium produced FAEE biodiesel at a titer of 4.8 mg/L under the optimum coculture conditions.
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