Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 159, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/61014
Keywords
Bioengineering; Issue 159; microbial engineering; yeast strains; single cell RNA sequencing; droplet microfluidics; high-throughput sequencing; hydrogel droplets
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Funding
- National Science Foundation Career Award [DBI-1253293]
- National Institutes of Health New Innovator Award [DP2AR068129, R01HG008978]
- National Science Foundation Technology Center [DBI-1548297]
- UCSF Center for Cellular Construction
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The powerful tools available to edit yeast genomes have made this microbe a valuable platform for engineering. While it is now possible to construct libraries of millions of genetically distinct strains, screening for a desired phenotype remains a significant obstacle. With existing screening techniques, there is a tradeoff between information output and throughput, with high-throughput screening typically being performed on one product of interest. Therefore, we present an approach to accelerate strain screening by adapting single cell RNA sequencing to isogenic picoliter colonies of genetically engineered yeast strains. To address the unique challenges of performing RNA sequencing on yeast cells, we culture isogenic yeast colonies within hydrogels and spheroplast prior to performing RNA sequencing. The RNA sequencing data can be used to infer yeast phenotypes and sort out engineered pathways. The scalability of our method addresses a critical obstruction in microbial engineering.
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