Journal
CHEMPHYSCHEM
Volume 17, Issue 16, Pages 2525-2534Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201600289
Keywords
circular dichroism; -peptides; membranes; membrane proteins; X-ray diffraction
Funding
- German Science Foundation DFG [SFB803]
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Transmembrane -peptides are promising candidates for the design of well-controlled membrane anchors in lipid membranes. Here, we present the synthesis of transmembrane -peptides with and without tryptophan anchors, as well as a novel iodine-labeled d-(3)-amino acid. By using one or more of the heavy-atom labeled amino acids as markers, the orientation of the helical peptide was inferred based on the electron-density profile determined by X-ray reflectivity. The -peptides were synthesized through manual Fmoc-based solid-phase peptide synthesis (SPPS) and reconstituted in unilamellar vesicles forming a right-handed 3(14)-helix secondary structure, as shown by circular dichroism spectroscopy. We then integrated the -peptide into solid-supported membrane stacks and carried out X-ray reflectivity and grazing incidence small-angle X-ray scattering to determine the -peptide orientation and its effect on the membrane bilayers. These -peptides adopt a well-ordered transmembrane motif in the solid-supported model membrane, maintaining the basic structure of the original bilayer with some distinct alterations. Notably, the helical tilt angle, which accommodates the positive hydrophobic mismatch, induces a tilt of the acyl chains. The tilted chains, in turn, lead to a membrane thinning effect.
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