Journal
BIOLOGY OPEN
Volume 9, Issue 4, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.049817
Keywords
Molecular cytogenetics; Mobile DNA; Karyotype evolution
Categories
Funding
- CAPES Scholarship in Aquatic Ecology and Fishing (PPGEAP), UFPA
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [475013/2012-3]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [Pro-amazonia-47/2012]
Ask authors/readers for more resources
Eukaryotic genomes exhibit substantial accumulation of repetitive DNA sequences. These sequences can participate in chromosomal reorganization events and undergo molecular cooption to interfere with the function and evolution of genomes. In turtles, repetitive DNA sequences appear to be accumulated at probable break points and may participate in events such as non-homologous recombination and chromosomal rearrangements. In this study, repeated sequences of 5S rDNA, U2 snRNA and Tc1/Mariner transposons were amplified from the genomes of the turtles, Podocnemis expanse and Podocnemis unifills, and mapped by fluorescence in situ hybridization. Our data confirm the 2n=28 chromosomes for these species (the second lowest 2n in the order Testudines). We observe high conservation of the co-located 5S rDNA and U2 snRNA genes on a small chromosome pair (pair 13), and surmise that this represents the ancestral condition. Our analysis reveals a wide distribution of the Tc1/Mariner transposons and we discuss how the mobility of these transposons can act on karyotypic reorganization events (contributing to the 2n decrease of those species). Our data add new information for the order Testudines and provide important insights into the dynamics and organization of these sequences in the chelonian genomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available