Impact of acute inflammation on Band 3 protein anion exchange capability in human erythrocytes

Objective: The impact of acute inflammation, revealed by C-reactive protein (CRP) plasma levels, has been studied on the erythrocytes anion exchanger Band 3 protein. Methods: Anion exchange capability through Band 3 protein, lipid peroxidation, -SH membrane groups and intracellular GSH levels have been measured on erythrocytes from patients with CRP >8 mg/L. Results: Under acute inflammation, a significant increase in anion exchange capability, increased lipid peroxidation, decreased-SH groups and GSH content were observed. Serum CRP levels recovery (after one week) was associated to -SH groups and GSH recovery, but not to anion exchange capability restoration. After 2 months, a total recovery of all parameters was observed. Conclusion: Band 3 protein anion exchange capability is affected by acute inflammation; the accelerated rate of anion exchange may be mainly due to lipid peroxidation, rather than to -SH groups oxidation; erythrocytes renewal could be needed to have a total recover of their function.

Automated summary

This study found that acute inflammation affects the function of erythrocytes, leading to increased anion exchange capability of the Band 3 protein, increased lipid peroxidation, and decreased -SH groups and GSH content. Serum CRP level recovery is associated with the recovery of -SH groups and GSH, but not with the restoration of anion exchange capability. After 2 months, all parameters were completely recovered.