Journal
JOURNAL OF NANOBIOTECHNOLOGY
Volume 18, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12951-020-00606-5
Keywords
Sciatic nerve injury; Autologous nerve grafting; Vascular endothelial growth factor (VEGF); Nerve growth factor (NGF); Gene therapy
Funding
- Nature Science Foundation of Shanghai, China [19ZR1439200]
- SUMHS Seed Foundation [HMSF-16-21-010]
- Science and Technology Development Foundation of Pudong New District, Shanghai, China [PKJ2016-Y55, PWZxq2017-03]
- Interdisciplinary Program of Shanghai Jiao Tong University [YG2017MS22, YG2017QN56]
- Translational Medicine Program of Shanghai Jiao Tong University [ZH2018QNA56]
- Program of Shanghai Sixth People's Hospital East Campus Foundation [2019YY001]
- Program of Shanghai Sixth People's Hospital Foundation [LY2Y0272]
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Background Peripheral nerve injury is one common clinical disease worldwide, in which sciatic nerve is anatomically the most challenging to regenerate given its length and large cross-sectional area. For the present, autologous nerve grafting remains to be the most ideal strategy when treating with sciatic nerve injury. However, this method sacrifices healthy nerves and requires highly intensive surgery, still calling for other advanced alternatives for nerve grafting. Results In this study, we utilized previously well-established gene delivery system to dually deliver plasmid DNA (pDNA) encoding vascular endothelial growth factor (VEGF) and nerve growth factor (NGF), exploring therapeutics for sciatic nerve injury. Low-molecular-weight branched polyethylenimine (bPEI) was constructed as the backbone structure of gene vectors, and it was further crosslinked to synthesize degradable polycations via the conjugation of dialdehydes. Potential synergistic effect between VEGF and NGF proteins were observed on rat sciatic nerve crush injury model in this study. Conclusions We concluded that dual delivery of plasmid VEGF and NGF as gene therapy could enhance sciatic nerve regeneration.
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