Journal
CHEMOSPHERE
Volume 144, Issue -, Pages 330-337Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2015.08.061
Keywords
Mercury; Hg resistance; Hg volatilization; Hg removal; Sphingobium
Categories
Funding
- University of South Australia
- Cooperative Research Centre for Contamination Assessment and Remediation of the Environment (CRC-CARE)
- Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia
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A mercury resistant bacterial strain, SA2, was isolated from soil contaminated with mercury. The 165 rRNA gene sequence of this isolate showed 99% sequence similarity to the genera Sphingobium and Sphingomonas of alpha-proteobacteria group. However, the isolate formed a distinct phyletic line with the genus Sphingobium suggesting the strain belongs to Sphingobium sp. Toxicity studies indicated resistance to high levels of mercury with estimated EC50 values 4.5 mg L-1 and 44.15 mg L-1 and MIC values 5.1 mg L-1 and 48.48 mg L-1 in minimal and rich media, respectively. The strain SA2 was able to volatilize mercury by producing mercuric reductase enzyme which makes it potential candidate for remediating mercury. ICP-QQQ-MS analysis of Hg supplemented culture solutions confirmed that almost 79% mercury in the culture suspension was volatilized in 6 h. A very small amount of mercury was observed to accumulate in cell pellets which was also evident according to ESEM-EDX analysis. The mercuric reductase gene merA was amplified and sequenced. The deduced amino acid sequence demonstrated sequence homology with a-proteobacteria and Ascomycota group. (C) 2015 Elsevier Ltd. All rights reserved.
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