Journal
PROTEIN & CELL
Volume 11, Issue 12, Pages 866-880Publisher
OXFORD UNIV PRESS
DOI: 10.1007/s13238-020-00727-5
Keywords
cell-cell communication; single-cell RNA sequencing; physical contact-dependent communication; chemical signal-dependent communication; ligand-receptor interaction; network biology
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Funding
- National Natural Science Foundation of China [81774153, 81973701]
- Natural Science Foundation of Zhejiang Province [LZ20H290002]
- National Youth Top-notch Talent Support Program [W02070098]
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For multicellular organisms, cell-cell communication is essential to numerous biological processes. Drawing upon the latest development of single-cell RNA-sequencing (scRNA-seq), high-resolution transcriptomic data have deepened our understanding of cellular phenotype heterogeneity and composition of complex tissues, which enables systematic cell-cell communication studies at a single-cell level. We first summarize a common workflow of cell-cell communication study using scRNA-seq data, which often includes data preparation, construction of communication networks, and result validation. Two common strategies taken to uncover cell-cell communications are reviewed, e.g., physically vicinal structure-based and ligand-receptor interaction-based one. To conclude, challenges and current applications of cell-cell communication studies at a single-cell resolution are discussed in details and future perspectives are proposed.
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