Journal
CHEMOSPHERE
Volume 149, Issue -, Pages 8-13Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2016.01.083
Keywords
miR-221; Lymphocyte; Benzene; Toxicology; Mechanisms
Categories
Funding
- Ministry of Environment Protection, P.R.C [201009008]
- Department of Science and Technology of Guangdong Province [2014A020212206]
- School of Public Health and Tropical Medicine of Southern Medical University, China [GW201403, GW201427]
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Benzene is a recognized environmental leukemogen, however, the mechanisms for its carcinogenesis have not been fully elucidated. Recently, miR-221, a suggested oncogene involved in a number of malignancies, has been detected with elevated expression levels in blood cells of patients with leukemia. To explore whether benzene exposure has an effect on the expression of miR-221, a population based cross-sectional study was conducted in southern China, with 97 petrol station attendants as the exposure group and 103 general residents as the control group. Plasma benzene was analyzed by using GC\MS. miR-221 in peripheral blood lymphocytes were measured by qRT-PCR and the Delta Ct value for each sample was calculated by normalizing the Ct value for miR-221 with U6 RNA (i.e., Delta Ct = Ct(miR-221) - Ct(U6)). Potential confounding factors were taken into account. Pearson correlation, univariate and multivariate logistic regression were performed in statistical analysis. The results showed that the air concentrations of benzene were significantly higher in petrol stations than in control sites (P < 0.05); The levels of benzene and miR-221 in exposure group were both significantly higher than in control group (P < 0.05) and there was a significant positive correlation between the two indexes (r = 0.851, P < 0.05); An association between benzene levels and the Delta Ct values for miR-221 was identified by univariate and multivariate logistic analysis (OR 0.274; 95%CI 0.117, 0.396). Our investigation indicates that benzene exposure may be related to elevated miR-221 expression in human lymphocytes. (C) 2016 Elsevier Ltd. All rights reserved.
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