Journal
CHEMPLUSCHEM
Volume 85, Issue 4, Pages 760-768Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.202000167
Keywords
cellular structural biology; dynamic nuclear polarization; in-cell NMR; magic angle spinning; solid-state NMR
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Solution-state NMR spectroscopy has become a powerful tool to study soluble proteins in cells, provided that they tumble sufficiently fast. In addition, cryo-electron tomography (cryo-ET) has recently displayed a tremendous potential to probe structures of large proteins and assemblies in their native cellular environments. However, challenges remain to obtain atomic-level information in native cell settings for proteins that are small, disordered, or are strongly engaged in intermolecular interactions. In this Minireview, we discuss recent progress in using sensitivity enhanced solid-state NMR spectroscopy methods in the context of cellular structural biology.
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