Journal
CELL REPORTS
Volume 31, Issue 3, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2020.107525
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Funding
- Intramural Research Program of the National Eye Institute (NEI), USA [ZIAEY000450, ZIAEY000456]
- NATIONAL EYE INSTITUTE [ZIAEY000450] Funding Source: NIH RePORTER
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Aging-associated functional decline is accompanied by alterations in the epigenome. To explore DNA modifications that could influence visual function with age, we perform whole-genome bisulfite sequencing of purified mouse rod photoreceptors at four ages and identify 2,054 differentially methylated regions (DMRs). We detect many DMRs during early stages of aging and in rod regulatory regions, and some of these cluster at chromosomal hotspots, especially on chromosome 10, which includes a longevity interactome. Integration of methylome to age-related transcriptome changes, chromatin signatures, and first-order protein-protein interactions uncover an enrichment of DMRs in altered pathways that are associated with rod function, aging, and energy metabolism. In concordance, we detect reduced basal mitochondrial respiration and increased fatty acid dependency with retinal age in ex vivo assays. Our study reveals age-dependent genomic and chromatin features susceptible to DNA methylation changes in rod photoreceptors and identifies a link between DNA methylation and energy metabolism in aging.
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