Journal
ACS SYNTHETIC BIOLOGY
Volume 9, Issue 5, Pages 1117-1128Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.9b00469
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Funding
- DTRA [HDTRA1-19-0021]
- NSF [1435957, 1807604, 1926793, 1805274, 1809436]
- National Institutes of Health [R21EB024102]
- Swedish Research council (VR) [VR-621-2014-5293]
- Swedish research council Formas [211-2013-70]
- [68122]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1435957] Funding Source: National Science Foundation
- Directorate For Engineering
- Div Of Electrical, Commun & Cyber Sys [1809436, 1926793, 1807604] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1805274] Funding Source: National Science Foundation
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There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples and cells, one can both electronically obtain a redox portrait of a biological system and, conversely, program gene expression. Here, we have created a cell-based synthetic biology-electrochemical axis in which engineered cells process molecular cues, producing an output that can be directly recorded via electronics-but without the need for added redox mediators. The process is robust; two key components must act together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. Catalytic transducer cells provide for signal-mediated surface expression of tyrosinase. Additionally, reagent transducer cells synthesize and export tyrosine, a substrate for tyrosinase. In cocultures, this system enables real-time electrochemical transduction of cell activating molecular cues. To demonstrate, we eavesdrop on quorum sensing signaling molecules that are secreted by Pseudomonas aeruginosa, N-(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.
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