Journal
BIOMEDICAL OPTICS EXPRESS
Volume 11, Issue 4, Pages 2213-2223Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.389435
Keywords
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Funding
- Japan Society for the Promotion of Science [19K09167, 19H03728]
- Grants-in-Aid for Scientific Research [19K09167, 19H03728] Funding Source: KAKEN
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We propose a line-field quantitative phase-imaging flow cytometer for analyzing large populations of label-free cells. Hydrodynamical focusing brings cells into the focus plane of an optical system while diluting the cell suspension, resulting in decreased throughput rate. To overcome the trade-off between throughput rate and in-focus imaging, our cytometer involves digitally extending the depth-of-focus on loosely hydrodynamically focusing cell suspensions. The cells outside the depth-of-focus range in the 70-mu m diameter of the core flow were automatically digitally refocused after image acquisition. We verified that refocusing was successful with our cytometer through statistical analysis of image quality before and after digital refocusing. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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