4.8 Article

An expanded library of orthogonal split inteins enables modular multi-peptide assemblies

Journal

NATURE COMMUNICATIONS
Volume 11, Issue 1, Pages -

Publisher

NATURE RESEARCH
DOI: 10.1038/s41467-020-15272-2

Keywords

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Funding

  1. UK Biotechnology and Biological Sciences Research Council [BB/N007212/1]
  2. UK Research and Innovation Future Leaders Fellowship [MR/S018875/1]
  3. Leverhulme Trust [RPG-2015-445]
  4. UK Engineering and Physical Sciences Research Council iCASE PhD scholarship
  5. Defence Science and Technology Laboratory
  6. BBSRC [BB/N007212/1] Funding Source: UKRI
  7. UKRI [MR/S018875/1] Funding Source: UKRI

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Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing activity of 34 inteins (both uncharacterized and known) using a rapid split fluorescent reporter characterization platform, and establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which can be simultaneously used in vitro. We show that orthogonal split inteins can be coupled to multiple split transcription factors to implement complex logic circuits in living organisms, and that they can also be used for the in vitro seamless assembly of large repetitive proteins with biotechnological relevance. Our work demonstrates the versatility and vast potential of an expanded library of orthogonal split inteins for their use in the fields of synthetic biology and protein engineering. Inteins allow the joining of protein segments through scarless ligation. Here the authors assess 34 inteins to establish a library of 15 mutually orthogonal split inteins for in vivo applications, 10 of which are also active in vitro.

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