Journal
NATURE COMMUNICATIONS
Volume 11, Issue 1, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-16154-3
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Funding
- National Key RAMP
- D Program of China [2019YFA0904900]
- National Natural Science Foundation of China [21978113]
- Key Technologies RAMP
- D Program of Jiangsu Province [BE2017622]
- Fundamental Research Funds for the Central Universities [JUSRP22031]
- National First-Class Discipline Program of Light Industry Technology and Engineering [LITE2018-08]
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Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 mu m(-1) and acetoin titer to 67.2g.L-1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 mu m(3) and poly(lactate-co-3-hydroxybutyrate) titer to 14.31g.L-1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories. Manipulation of genes controlling microbial shapes can affect bio-production. Here, the authors employ an optogenetic method to realize dynamic morphological engineering of E. coli replication and division and show the increased production of acetoin and poly(lactate-co-3-hydroxybutyrate).
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