Journal
NATURE COMMUNICATIONS
Volume 11, Issue 1, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-020-16514-z
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Funding
- Natural Science Foundation of China [31930021, 31970633, 31671215, 31471125, 31671360, 31801095]
- Special Project on Precision Medicine under the National Key RD Program [2017YFC0906600, 2018YFC0910500]
- National Program for Support of Top-Notch Young Professionals
- program for HUST Academic Frontier Youth Team
- Fundamental Research Funds for the Central Universities [2019kfyRCPY043]
- Young Changjiang Scholars Program of China
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Most organisms on the earth exhibit circadian rhythms in behavior and physiology, which are driven by endogenous clocks. Phosphorylation plays a central role in timing the clock, but how this contributes to overt rhythms is unclear. Here we conduct phosphoproteomics in conjunction with transcriptomic and proteomic profiling using fly heads. By developing a pipeline for integrating multi-omics data, we identify 789 (similar to 17%) phosphorylation sites with circadian oscillations. We predict 27 potential circadian kinases to participate in phosphorylating these sites, including 7 previously known to function in the clock. We screen the remaining 20 kinases for effects on circadian rhythms and find an additional 3 to be involved in regulating locomotor rhythm. We re-construct a signal web that includes the 10 circadian kinases and identify GASKET as a potentially important regulator. Taken together, we uncover a circadian kinome that potentially shapes the temporal pattern of the entire circadian molecular landscapes.
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