Journal
EPIGENOMICS
Volume 12, Issue 8, Pages 701-713Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/epi-2019-0391
Keywords
C2C12; circadian; epigenetics; methylation; muscle; myotube; transcriptomics
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Funding
- Novo Nordisk Foundation [NNF18CC003490]
- Novo Nordisk Foundation Challenge Grant [NNF14OC0011493]
- Swedish Research Council [2015-00165]
- Swedish Research Council [2015-00165] Funding Source: Swedish Research Council
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Aim: Innate circadian rhythms are critical for optimal tissue-specific functions, including skeletal muscle, a major insulin-sensitive tissue responsible for glucose homeostasis. We determined whether transcriptional oscillations are associated with CpG methylation changes in skeletal muscle. Materials & methods: We performed rhythmicity analysis on the transcriptome and CpG methylome of circadian synchronized myotubes. Results: We identified several transcripts and CpG-sites displaying oscillatory behavior, which were enriched with Gene Ontology terms related to metabolism and development. Oscillating CpG methylation was associated with rhythmic expression of 31 transcripts. Conclusion: Although circadian oscillations may be regulated by rhythmic DNA methylation, strong rhythmic associations between transcriptome and CpG methylation were not identified. This resource constitutes a transcriptomic/epigenomic atlas of skeletal muscle and regulation of circadian rhythms.
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