Journal
VIRUSES-BASEL
Volume 12, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/v12050573
Keywords
crAssphage; genome; diversity-generating retroelement (DGR); variable repeat; template repeat; initiation of mutagenic homing
Categories
Funding
- Russian Science Foundation [18-74-00082]
- Russian State-funded budget project of ICBFM SB RAS [AAAA-A17-117020210027-9]
- Russian Science Foundation [18-74-00082] Funding Source: Russian Science Foundation
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A new crAss-like genome encoding diversity-generating retroelement (DGR) was found in the fecal virome of a healthy volunteer. The genome of the phage referred to as the crAssphage LMMB, belonged to the candidate genus I of the AlphacrAssvirinae subfamily. The DGR-cassette of the crAssphage LMMB contained all the essential elements: the gene encoding reverse transcriptase (RT), the target gene (TG) encoding the tail-collar fiber protein, and variable and template repeats (VR and TR) with IMH (initiation of mutagenic homing) and IMH* sequences at the 3 ' -end of the VR and TR, respectively. Architecture of the DGR-cassette was TG-VR(IMH)-TR(IMH*)-RT and an accessory variable determinant (avd) was absent from the cassette. Analysis of 91 genomes and genome fragments from genus I of the AlphacrAssvirinae showed that 15 (16%) of the genomes had DGRs with the same architecture as the crAssphage LMMB, while 66 of the genomes contained incomplete DGR-cassettes or some elements of the DGR.
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