Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 22, Issue 36, Pages 12660-12664Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.201602489
Keywords
fluorescent probes; imaging; nucleolus; silver nanodots; specific staining
Categories
Funding
- Korean National Research Foundation [2013R1A1A2061063, 2015R1A2A1A15055721]
- National Research Foundation of Korea [2015R1A2A1A15055721, 2013R1A1A2061063] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Metal complexes are frequently used for biological applications due to their special photophysical and chemical characteristics. Due to strong interactions between metals and biomacromolecules, a random staining of cytoplasm or nucleoplasm by the complexes results in a low signal-to-background ratio. In this study, we used luminescent silver nanodots as a model to investigate the major driving force for non-specific staining in cellular matrices. Even though some silver nanodot emitters exhibited excellent specific staining of nucleoli, labeling with nanodots was problematic owing to severe non-specific staining. Binding between silver and sulfhydryl group of proteins appeared to be the major factor that enforced the silver staining. The oxidation of thiol groups in cells with hexacyanoferrate(III) dramatically weakened the silver-cell interaction and consequently significantly improved the efficiency of targeted staining.
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