Journal
TRANSBOUNDARY AND EMERGING DISEASES
Volume 67, Issue 4, Pages 1485-1491Publisher
WILEY
DOI: 10.1111/tbed.13620
Keywords
antibody; clustered regularly interspaced short palindromic repeats (CRISPR); Corona Virus Disease 2019 (COVID-19); detection; real-time RT-PCR; reverse transcription loop-mediated isothermal amplification (RT-LAMP); severe acute respiratory syndrome coronavirus 2 (2019 Novel Coronavirus SARS-CoV-2)
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Funding
- National Natural Science Foundation of China [31772747]
- Science and Technology Research Program during the 13th Five-Year Plan Period of Jilin Educational Committee [JJKH20190172KJ]
- National Key Research and Development Program of China [2017YFD0500103]
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Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. Therefore, convenient, timely and accurate detection of SARS-CoV-2 is urgently needed. Here, we review the types, characteristics and shortcomings of various detection methods, as well as perspectives for the SARS-CoV-2 diagnosis. Clinically, nucleic acid-based methods are sensitive but prone to false-positive. The antibody-based method has slightly lower sensitivity but higher accuracy. Therefore, it is suggested to combine the two methods to improve the detection accuracy of COVID-19.
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