Journal
TALANTA
Volume 211, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.120680
Keywords
Digital PCR; Microfluidic impact printing; Genetic analyses
Categories
Funding
- National Natural Science Foundation of China [51675505]
- Joint Research Fund for Overseas Chinese Scholars and Scholars in Hong Kong and Macao [51929501]
- Program for Guangdong Introducing Innovative and Entrepreneurial Teams [2016ZT06D631]
- Shenzhen Fundamental Research Program [JCYJ20170413164102261]
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Digital PCR enabled high-sensitivity and quantitative measurements of rare biological variants. A new digital droplet-enabled PCR technology was introduced in this paper, which partitioned genetic targets into a planar nanoliter droplet array by using a microfluidic impact printer (MIP) with a disposable microfluidic chip. The accuracy of this MIP-enabled PCR technology was verified by detecting a series of concentration gradients of GAPDH gene across spanning four orders of magnitude (from 0.464 copies/mu L to 464 copies/mu L). Furthermore, this technology was applied to detect the expressions of p53 gene in colon cancer tissues and adjacent nontumorous tissues, from which the copies of the nucleic acids could be absolute-quantitatively determined. The outcomes were consistent with the results of using the conventional real-time PCR, demonstrating a great potential of the MIP-enabled digital PCR in detecting gene expression in clinical samples.
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