Journal
TALANTA
Volume 211, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2019.120707
Keywords
Platinum nanoparticles; Nanozyme; Oxidase-like activity; Colorimetric sensor; Isoniazid; Drug tablet
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Funding
- National Natural Science Foundation of China [21175023, 21675024]
- Program for Innovative Leading Talents in Fujian Province [2016B016]
- Program for Innovative Research Team in Science and Technology in Fujian Province University [2018B033]
- Science and Technology Project of Fujian Province [2018L3008]
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In this study, a colorimetric sensing assay of isoniazid based on excellent oxidase-like activity of heparin sodium stabilized platinum nanoparticles (HS-PtNPs) has been demonstrated. The newly prepared HS-PtNPs exhibit a great dispersion with an average size distribution of 4.8 +/- 0.6 nm, and maintain more than 90% catalytic activity under strong acid and alkali or long-term storage conditions, indicating a robust nanomaterial with attractive potential. The HS-PtNPs show distinct oxidase-like activity with an ultrahigh affinity (Km = 0.01012 mm) for 3, 3', 5, 5' tetramethylbenzidine (TMB). More significantly, we found that the pyridine ring of isoniazid has a strong reductive hydrazyl substitution, which can compete with TMB for the catalytic site of HS-PtNPs resulting in a colorless solution. Accordingly, a colorimetric sensing of isoniazid was fabricated. A linear relationship for isoniazid was achieved in 2.5 x 10(-6) to 2.5 x 10(-4) M (R-2 = 0.998) with a low limit of detection 1.7 x 10(-6) M (S/N = 3). Recovery experiments in drug tablets show that the standard recovery rates were 95%-103%. The quantitative detection data for isoniazid in drug tablets calculated respectively from the standard method and this method exhibited a high correlation coefficient (a slope of 0.9995), suggesting that high accuracy in isoniazid detection.
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