Journal
STEM CELLS AND DEVELOPMENT
Volume 29, Issue 9, Pages 586-598Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2019.0176
Keywords
cell therapy; stem cells; mesenchymal stromal cells; Wharton's jelly; hemorrhagic stroke; intracerebral hemorrhage
Funding
- Departamento de Ciencia e Tecnologia (DECIT) do Ministerio da Saude
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [402319/2013-3]
- Instituto Nacional de Ciencia e Tecnologia em Medicina Regenerativa
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88887.136364/2017-00, PROBITEC 2262/2012 -23038.004161/2018-88]
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) [19/2016]
Ask authors/readers for more resources
Intracerebral hemorrhage (ICH) is as a life-threatening condition that can occur in young adults, often causing long-term disability. Recent preclinical data suggest mesenchymal stromal cell (MSC)-based therapies as promising options to minimize brain damage after ICH. However, therapeutic evidence and mechanistic insights are still limited, particularly when compared with other disorders such as ischemic stroke. Herein, we employed a model of collagenase-induced ICH in young adult rats to investigate the potential therapeutic effects of an intravenous injection of human umbilical cord Wharton's jelly-derived MSCs (hUC-MSCs). Two doses of collagenase were used to cause moderate or severe hemorrhages. Magnetic resonance imaging showed that animals treated with hUC-MSCs after moderate ICH had smaller residual hematoma volumes than vehicle-treated rats, whereas the cell therapy failed to decrease the hematoma volume in animals with a severe ICH. Functional assessments (rotarod and elevated body swing tests) were performed for up to 21 days after ICH. Enduring neurological impairments were seen only in animals subjected to severe ICH, but the cell therapy did not induce statistically significant improvements in the functional recovery. The biodistribution of Technetium-99m-labeled hUC-MSCs was also evaluated, showing that most cells were found in organs such as the spleen and lungs 24 h after transplantation. Nevertheless, it was possible to detect a weak signal in the brain, which was higher in the ipsilateral hemisphere of rats subjected to a severe ICH. These data indicate that hUC-MSCs have moderately beneficial effects in cases of less severe brain hemorrhages in rats by decreasing the residual hematoma volume, and that optimization of the therapy is still necessary.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available