Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 240, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.118555
Keywords
Coumarin derivative; Lysosome-targeted; Fluorescent probe; Specific detection; Dual-color mode imaging
Categories
Funding
- NSFC [21472067, 21672083, 21877048]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004]
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Biothiols, as part of the reactive sulfur species (RSS), are a class of bioactive molecules that play important physiological roles in human body. However, due to the similarity in structure and reaction sites of biothiols, it is difficult to differentiated detection them at the same time. In this work, a fluorescent probe CM-NBD combined coumarin derivative and 7-nitrobenzofurazan has been developed, which can effectively detect biothiols through simple ether cleavage. Because of a specific location group, CM-NBD canwell localize in lysosomes with a high colocalization coefficient. Interesting, due to theweakly acidic environment of lysosomes, Cys can be distinguished from Hcy/GSH and H2S via dual-color mode. The probe is able not only to image exogenous biothiols but also to discriminate Cys from Hcy/GSH and H2S in cells and zebrafish model. (c) 2020 Elsevier B.V. All rights reserved.
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