Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 231, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.118141
Keywords
Reactive sulfur species; Ratiometric; Colorimetric; Living cell imaging
Categories
Funding
- Scientific Research Foundation of Fujian Normal University in China [IRTL1703]
- Innovative Research Teams Program II of Fujian Normal University in China [IRTL1703]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (SRF for ROCS, SEM)
- Natural Science Foundation of Fujian Province, China [2016J01143]
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RSSs (reactive sulfur species) and their metabolites, such as H2S, S-n(2-),SO32-/HSO3-,S2O42- and S2O52- (Reactive Inorganic Sulfur Species, RISSs), play a crucial role in the cushion against oxidative stress and the other physiological events. The molecular mechanisms how they affect cellular signaling and other physiological events remain largely unknown. To address their physiological functions, the techniques that can track their levels should be invaluable. Herein, six coumarin hemicyanine scaffolds (CH-RISSs) were synthesized and their fast and strong re- sponses upon H2S, S-n(2-), SO32-, HSO3- S2O42- and S2O52- (Reactive Inorganic Sulfur Species, RISSs) were clarified in the absorption (colorimetric) and fluorescence (ratiometric) spectra, which showed good stability in the physiological pH (7.4). Upon the analytes, the maxima absorption of CH-RISSs switched from similar to 580 nm to similar to 400 nm in the absorption spectra. The fluorescence of CH-RISSs depleted at 650-660 nm and increased at 480-505 nm upon the RISSs. Both of coumarin hemicyanine structures with C12 alkyl chain (CH-RISS-3 and CH-RISS-6) showed quick and robust ratiometric fluorescence switch in the living cell imaging. Access to the fluorescent probes for RISSs sets the stage for applying the developing technologies to probe reactive sulfur biology in living systems. (C) 2020 Published by Elsevier B.V.
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