Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 309, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.127731
Keywords
Fluorescent probe; Mitochondria-targeting; Hydrogen peroxide; Para-nitrophenyl oxoacetyl; Large Stokes shift
Funding
- Beijing Key Laboratory of Environmental Viral Oncology
- Beijing Key Laboratory for Green Catalysis and Separation
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Hydrogen peroxide (H2O2) is a small molecule of reactive oxygen species, mainly produced by cell mitochondria, which plays an important role in physiological processes. Excess of H2O2 in normal cells could causes a variety of diseases. For that reason, it is very significant to emerge a detection method with high-precision for H2O2. Herein, we reported a structurally novel fluorescent probe, para-nitrophenyl oxoacetyl rhodamine (RhB-NIR) for the detection of H2O2 levels in living cells. The results show that the Stokes shift for RhB-NIR is 140 nm, much larger than those for other reported rodamine derivatives. RhB-NIR shows satisfactory specificity and high sensitivity for the detection of H2O2. Excellent linear relationship with H2O2 within a certain concentration range (0-10 mu M) was displayed, and the limit of detection (LOD) is 61 nM. More importantly, RhB-NIR realized fluorescence imaging of H2O2 in living cells. In addition, RhB-NIR also shows great potential for targeting mitochondria in cell colocalization experiments, in which the Pearson's coefficient is 0.91 using Rhodamine 123 as a reference. This probe will be a promising tool for detecting H2O2 in the future research.
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