Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 308, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.127687
Keywords
PtNi@Cu-TCPP(Fe) hybrid nanosheets; Electro-catalysis; Electrochemical immunosensor; CALP
Funding
- National Science and Technology Major Project [2018ZX10101003-001-009]
- National Natural Science Foundation of China [81960002, 81873980, 81560002]
- National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX10732202]
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In this work, a novel high electro-catalytic material was synthesized using PtNi nanospheres to functionalize two-dimensional (2D) ultrathin Cu-TCPP(Fe) nanosheets (PtNi@Cu-TCPP(Fe)), and was further applied to develop an enzyme-free electrochemical immunosensor for the ultrasensitive determination of calprotectin (CALP). The bimetallic Cu-TCPP(Fe) nanosheets with large surface area and massive accessible active sites permitted multiple PtNi to attach their surface, which not only enhanced the catalytic ability and conductivity for nonenzymatic amplification but also provided the modified active sites for antibody immobilization as signal labels. Upon the dual electro-catalytic activity of Cu-TCPP(Fe) and PtNi towards H2O2 reduction for signal amplification, this proposed sandwich CALP immunosensor exhibited a wide linear range of 200 fg mL(-1)similar to 50 ng mL(-1) and a low detection limit of 137.7 fg mL(-1). The functionalized MOF nanosheets-based biosensing method offered an enzyme-free signal amplification strategy, which provided great promise for further bioanalysis and clinical study.
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