Journal
SENSORS
Volume 20, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/s20102924
Keywords
microbiosensor; glutamate; glutamate oxidase; Prussian blue nanocubes; chitosan; amperometry
Funding
- National Key Research and Development Program of China [2017YFE019550, 2017YFC1308501]
- Fundamental Research Funds for the Central Universities [2019QNA5027, K20200185]
- National Natural Science Foundation of China [31627802, 81571769]
- Zhejiang Laboratory [2018EB0ZX01]
- Zhejiang University Education Foundation Global Partnership Fund
- Zhejiang Province Key RD Programs [2018C01037, 2020C03039]
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A simple method that relies only on an electrochemical workstation has been investigated to fabricate a highly sensitive glutamate microbiosensor for potential neuroscience applications. In this study, in order to develop the highly sensitive glutamate electrode, a 100 mu m platinum wire was modified by the electrochemical deposition of gold nanoparticles, Prussian blue nanocubes, and reduced graphene oxide sheets, which increased the electroactive surface area; and the chitosan layer, which provided a suitable environment to bond the glutamate oxidase. The optimization of the fabrication procedure and analytical conditions is described. The modified electrode was characterized using field emission scanning electron microscopy, impedance spectroscopy, and cyclic voltammetry. The results exhibited its excellent sensitivity for glutamate detection (LOD = 41.33 nM), adequate linearity (50 nM-40 mu M), ascendant reproducibility (RSD = 4.44%), and prolonged stability (more than 30 repetitive potential sweeps, two-week lifespan). Because of the important role of glutamate in neurotransmission and brain function, this small-dimension, high-sensitivity glutamate electrode is a promising tool in neuroscience research.
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