Journal
POULTRY SCIENCE
Volume 99, Issue 8, Pages 3979-3986Publisher
ELSEVIER
DOI: 10.1016/j.psj.2020.04.024
Keywords
selenide chitosan sulfate; glutathione; glutathione peroxidase; hepatocyte; chicken
Categories
Funding
- Shandong Major Scientific and Technological Innovation Project [2019JZZY020611]
- Shandong Province Higher Educational Science and Technology Program [J18KA131, J18KA143]
- Chinese National Natural Science Foundation [31001093, 31101867]
- Shandong modern agricultural industry technology system poultry innovation team projects [SDAIT-11-07]
- one-three-one Program of Innovative Talents Training of Tianjin
- Young and Middle aged Backbone Innovative Talents Training Program of Higher Educational Institutions of Tianjin
- Tianjin Research Program of Application Foundation and Advanced Technology [15JCYBJC30600]
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This study aimed to investigate the effects of selenide chitosan sulfate (Se-CTS-S) on glutathione (GSH) system in hepatocytes and chickens. Chitosan, sodium selenite (Na2SeO3), selenide chitosan, chitosan sulfate (CTS-S), and Se-CTS-S were added to the culture medium and the basal diets; glutathione peroxidase (GSH-Px) activity, GSH content, total antioxidant capacity (T-AOC), and mRNA levels of cellular GPx (GPx-1) and phospholipid hydroperoxide GPx (GPx-4) in vivo and in vitro were determined. The results showed that Se-CTS-S increased (P < 0.05) GPx-1 and GPx-4 mRNA levels in hepatocytes and livers, and GSH-Px activity, GSH content, and T-AOC in the medium, hepatocytes, plasma, and livers compared with the control and chitosan treatments. Compared with CTS-S, Se-CTS-S treatments increased (P < 0.05) GPx-1 and GPx-4 mRNA levels in hepatocytes and livers, and GSH-Px activity, GSH content, and T-AOC capacity in the medium, hepatocytes, and livers. Compared with Na2SeO3 and CTS-Se, Se-CTS-S increased (P < 0.05) GPx-1 mRNA levels in hepatocytes and livers, GPx-4 mRNA levels in hepatocytes and livers, GSH-Px activity in the medium, hepatocytes, and livers, GSH contents in plasma and livers, and T-AOC in the medium, plasma, and livers. Thus, Se-CTS-S showed better biological activity that mainly benefited from the synergistic effects of Se and sulfate on GSH system.
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