Journal
PLASMA PROCESSES AND POLYMERS
Volume 17, Issue 10, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.201900257
Keywords
atmospheric pressure plasma; Ca2+ signaling; peroxynitrite; plasma medicine; TRP channel
Funding
- Research Institute of Electrical Communication, Tohoku University
- Japan Society for the Promotion of Science [15J01427, 16K12863, 16K13708, 18H03687, 19K14698, 24108004]
- Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
- Grants-in-Aid for Scientific Research [16K12863, 16K13708, 15J01427, 19K14698] Funding Source: KAKEN
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Although the externally controllable extracellular supply of the short-lived reactive oxygen and nitrogen species, such as O-2(center dot-), (NO)-N-center dot, and ONOO-, could potentially manipulate cellular functions, their simple administration to cells is likely to be ineffective due to their rapid deactivation. In this study, we found a method of a continuous supply of O-2(center dot-)/ONOO- over a few minutes, which is triggered by irradiation of a nonequilibrium atmospheric pressure plasma to commonly used organic buffers (e.g., 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, HEPES). In addition, a continuous low-dose O-2(center dot-)/ONOO- supply was shown to induce a physiologically relevant Ca2+ response and subsequently the uptake of a large cation mediated by transient receptor potential channel family member(s). Our results provide a novel approach to the continuous O-2(center dot-)/ONOO- supply, requiring controllable and mass-volume treatments.
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