Journal
PLANTA MEDICA
Volume 86, Issue 10, Pages 674-685Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/a-1161-0372
Keywords
maca; Lepidium meyenii; Brassicaceae; flow injection mass spectrometry; metabolite profiling; imidazoles; macamides; glucosinolates; PCA
Categories
Funding
- Agricultural Research Service of the U.S. Department of Agriculture
- Office of Dietary Supplements, National Institute of Health, Department of Health and Human Services
Ask authors/readers for more resources
Maca (Lepidium meyenii, synonymL. peruvianum) was analyzed using a systematic approach employing principal component analysis of flow injection mass spectrometry fingerprints (no chromatographic separation) to guide the selection of samples for metabolite profiling and DNA next generation sequencing. Samples consisted of 39 commercial maca supplements from 11 manufacturers, 31 unprocessed maca tubers grown in Peru and China, and a historic non-tuber maca sample from Peru. Principal component analysis of flow injection mass spectrometry fingerprints initially placed all the maca samples in three classes with similar chemical composition: commercial maca samples, tubers grown in Peru, and tubers grown in China. Metabolite profiling identified 67 compounds in the negative mode and 51 compounds in the positive mode. Compounds identified by metabolite profiling (macamides, glucosinolates, amino acids, fatty acids, polyunsaturated fatty acids, saccharides, imidazoles) were then used to identify ions in the flow injection mass spectrometry fingerprints. The tuber fingerprints were analyzed by factorial multivariate analysis of variance revealing that black, red, and yellow maca from Peru and black and yellow maca from China were compositionally different with respect to color and country. Critical ions were identified that allowed for the differentiation of maca between colors from the same country or between two countries with the same color. Genetically, all samples were confirmed to beL. meyeniibased on next generation sequencing at three gene regions (ITS2,psbA, andtrnL) and comparison to recorded sequences of vouchered standards.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available