4.7 Article

Identifying Plasmopara viticola resistance Loci in grapevine (Vitis amurensis) via genotyping-by-sequencing-based QTL mapping

Journal

PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 154, Issue -, Pages 75-84

Publisher

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2020.05.016

Keywords

Grapevine; Vitis amurensis; Plasmopara viticola resistance; Genotyping-by-sequencing; Quantitative trait locus; Mapping

Categories

Funding

  1. National Key Research & Development Program of China [2018YFD1000301]
  2. China Agriculture Research System [CARS-29-yc-2]
  3. Shanghai Municipal Commission for Science and Technology [18391900400]
  4. Shanghai Municipal Agricultural Commission [2018-02-08-00-08-F01552]
  5. Shanghai Jiao Tong University [WF107115001]
  6. Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences [2015YT95]

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Downy mildew, caused by Plasmopara viticola, is a major disease that affects grapevines, and a few resistance (R) genes have been identified thus far. In order to identify R genes, we investigated F-1 progeny from a cross between the downy mildew-resistant Vitis amurensis 'Shuang Hong' and the susceptible Vitis vinifera 'Cabernet Sauvignon'. The P. viticola-resistance of the progeny varied continuously and was segregated as a quantitative trait. Genotyping-by-sequencing was used to construct linkage maps. The integrated map spanned 1898.09 cM and included 5603 single nucleotide polymorphisms on 19 linkage groups (LGs). Linkage analysis identified three quantitative trait loci (QTLs) for P. viticola resistance: 22 (Rpv22) on LG 02, Rpv23 on LG15, and Rpv24 on LG18. The phenotypic variance contributed by these three QTLs ranged from 15.9 to 30.0%. qRT-PCR analysis of R-gene expression in these QTLs revealed a CC-NBS-LRR disease resistance gene RPP8, two LRR receptor-like serine/threonine-protein kinases, a serine/threonine-protein kinase BLUS1, a glutathione peroxidase 8, an ethylene-responsive transcription factor ERF038, and a transcription factor bZIP11 were induced by P. viticola, and these genes may play important role in P. viticola response.

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