Uncovering PGPB Vibrio spartinae inoculation-triggered physiological mechanisms involved in the tolerance of Halimione portulacoides to NaCl excess

Plant growth promoting bacteria' (PGPB) beneficial role on plant tolerance to salinity stress has previously been well recognized. However, bacteria-triggered plant physiological mechanisms involved in this response require investigation, especially in plants with innate salt tolerance. A glasshouse experiment was designed to investigate the effect of the PGPB Vibrio spartinae on Halimione portulacoides growth, physiological performance and ion homeostasis in plants exposed to 0, 171, 510 and 1020 mM NaCl for 100 days. Bacterial inoculation alleviated similar to 28% of the deleterious impact of salinity excess on the relative growth rate (RGR) in plants grown at 510 mM and led to 30% and 44% enhancements in those exposed to 0 and 171 mM NaCl, respectively. This effect was linked to a reduction in Na tissue concentrations which improved plant ion homeostasis at elevated NaCl concentration, and to the overall protective effects on various steps in the photosynthetic pathway between 0 and 510 mM NaCl. Thus, inoculated plants were able to maintain higher net photosynthesis (A(N)) than their non-inoculated counterparts. Hence, A(N) differences under saline conditions were ascribed to inoculation amelioration NaCl-induced CO2 diffusion limitations, as reflected in the greater g(s) and C-i values recorded at 171 and 510 mM NaCl, together with an enhancement of photochemical apparatus functionality (in terms of energy absorption, transformation and transport), as indicated by a higher electron transport rate (ETR) and energy fluxes derived from Kautsky curves, compared with their non-inoculated counterparts.