Journal
PLANT AND CELL PHYSIOLOGY
Volume 61, Issue 8, Pages 1507-1516Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcaa071
Keywords
Autoimmunity; DEAD-box RNA helicase; MAP kinase pathway; Nuclear RNA exosome; Posttranscriptional gene regulation; Pseudomonas syringae pv. tomato DC3000
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Funding
- Gatsby Charitable Foundation
- UK Biotechnology and Biological Sciences Research Council
- Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowships for Research Abroad
- JSPS KAKENHI [16H06279, 19770045, 22780037, 25450060, 19K06054]
- Next Generation Leading Research Fund for 2017 of Kagawa University Research Promotion Program (KURPP)
- Next Generation Leading Research Fund for 2018 of Kagawa University Research Promotion Program (KURPP)
- Cooperative Research Grant of the Genome Research for BioResource, NODAI Genome Research Center, Tokyo University of Agriculture
- Sasakawa Scientific Research Grant from The Japan Science Society
- Grants-in-Aid for Scientific Research [22780037, 19770045, 25450060, 19K06054] Funding Source: KAKEN
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In Arabidopsis thaliana, a mitogen-activated protein kinase pathway, MEKK1-MKK1/MKK2-MPK4, is important for basal resistance and disruption of this pathway results in dwarf, autoimmune phenotypes. To elucidate the complex mechanisms activated by the disruption of this pathway, we have previously developed a mutant screening system based on a dwarf autoimmune line that overexpressed the N-terminal regulatory domain of MEKK1. Here, we report that the second group of mutants, smn2, had defects in the SMN2 gene, encoding a DEAD-box RNA helicase. SMN2 is identical to HEN2, whose function is vital for the nuclear RNA exosome because it provides non-ribosomal RNA specificity for RNA turnover, RNA quality control and RNA processing. Aberrant SMN1/RPS6 transcripts were detected in smn2 and hen2 mutants. Disease resistance against Pseudomonas syringae pv. tomato DC3000 (hopA1), which is conferred by SMN1/RPS6, was decreased in smn2 mutants, suggesting a functional connection between SMN1/RPS6 and SMN2/HEN2. We produced double mutants mekklsmn2 and mpk4smn2 to determine whether the smn2 mutations suppress the dwarf, autoimmune phenotypes of the rnekkl and mpk4 mutants, as the smnl mutations do. As expected, the mekkl and mpk4 phenotypes were suppressed by the smn2 mutations. These results suggested that SMN2 is involved in the proper function of SMN1/RPS6. The Gene Ontology enrichment analysis using RNA-seq data showed that defense genes were downregulated in smn2, suggesting a positive contribution of SMN2 to the genome-wide expression of defense genes. In conclusion, this study provides novel insight into plant immunity via SMN2/HEN2, an essentiol component of the nuclear RNA exosome.
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