Journal
CHEMICO-BIOLOGICAL INTERACTIONS
Volume 258, Issue -, Pages 108-114Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.cbi.2016.08.022
Keywords
5-Bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB); Proinflammatory; RAW 264.7 cells; MAPKs; NF-kappa B
Funding
- Marine Biotechnology Program - Ministry of Oceans and Fisheries of Korean Government [PM59530, 20150220]
- Korea Institute of Ocean Science and Technology, Republic of Korea [PE99411]
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The aim of the present study was to investigate the effects of 5-bromo-2-hydroxy-4-methyl-benzaldehyde (BHMB) on inflammatory responses to lipopolysaccharide (LPS) in RAW 264.7 cells and the associated mechanism of action. BHMB concentration-dependently suppressed protein and mRNA expressions of iNOS and COX-2, thereby inhibiting the production of NO and PGE(2) in LPS-stimulated RAW 264.7 cells. BHMB also reduced the mRNA expression of TNF-alpha, IL-6, and IL-1 beta in LPS-stimulated RAW 264.7 cells. To elucidate the mechanism underlying the anti-inflammatory activity of BHMB, we investigated the effects of BHMB on the mitogen-activated protein kinase and nuclear factor-kappa B (NF-kappa B) pathways. BHMB suppressed the phosphorylation and degradation of I kappa B-alpha and markedly inhibited the nuclear translocation of p65 and p50 in LPS-stimulated RAW 264.7 cells. The compound also inhibited the LPS-stimulated phosphorylation of ERK and p38. Taken together, these results illustrated that BHMB suppresses pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 cells by inhibiting the phosphorylation of ERK and p38 and the activation of NF-kappa B. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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