Journal
ORGANIC LETTERS
Volume 22, Issue 9, Pages 3444-3448Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.0c00892
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Funding
- National Key R&D Program of China [2018YFA0901602]
- National Natural Science Foundation of China [1602246, 21778072]
- Tianjin Municipal Science and Technology Commission [15PTCYSY00020]
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In this study, an engineered carbonyl reductase (M4) was obtained through structure-guided directed evolution of a carbonyl reductase (SSCR) from Sporobolomyces salmonicolor AKU4429. Mutant M4 showed 23.9-fold enhancement of enzyme activity toward the model substrate 2-methyl-2-benzyl-1,3-cyclo-pentanedione, affording the (2S,3S)-stereoisomer in >98% ratio. This variant also showed excellent stereoselectivity toward most of the tested substrates, offering a valuable biocatalyst for the stereoselective reduction of these cyclic diketones to access the corresponding (2S,3S)-2,2-disubstituted-3-hydroxyketones.
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