Journal
OPTICS LETTERS
Volume 45, Issue 8, Pages 2247-2250Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.389300
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- Regione Toscana
- H2020 LEIT Information and Communication Technologies [654148, 732111]
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We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features. (C) 2020 Optical Society of America
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