Journal
OPTICS EXPRESS
Volume 28, Issue 10, Pages 15663-15677Publisher
OPTICAL SOC AMER
DOI: 10.1364/OE.391016
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Funding
- National Institute of General Medical Sciences [R01 GM128214, R01 GM132860]
- National Science Foundation [1904684]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1904684] Funding Source: National Science Foundation
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Being able to image chemical bonds with high sensitivity and speed, stimulated Raman scattering (SRS) microscopy has made a major impact in biomedical optics. However, it is well known that the standard SRS microscopy suffers from various backgrounds, limiting the achievable contrast, quantification and sensitivity. While many frequency-modulation (FM) SRS schemes have been demonstrated to retrieve the sharp vibrational contrast, they often require customized laser systems and/or complicated laser pulse shaping or introduce additional noise, thereby hindering wide adoption. Herein we report a simple but robust strategy for FM-SRS microscopy based on a popular commercial laser system and regular optics. Harnessing self-phase modulation induced self-balanced spectral splitting of picosecond Stokes beam propagating in standard single-mode silica fibers, a high-performance FM-SRS system is constructed without introducing any additional signal noise. Our strategy enables adaptive spectral resolution for background-free SRS imaging of Raman modes with different linewidths. The generality of our method is demonstrated on a variety of Raman modes with effective suppressing of backgrounds including non-resonant cross phase modulation and electronic background from two-photon absorption or pump-probe process. As such, our method is promising to be adopted by the SRS microscopy community for background-free chemical imaging. (c) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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