Journal
OPTICS AND LASERS IN ENGINEERING
Volume 128, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.optlaseng.2020.106003
Keywords
Fourier ptychographic diffraction tomography; Three-dimensional microscopy; Phase retrieval; Refractive index
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Funding
- National Natural Science Foundation of China [61722506, 11574152]
- Outstanding Youth Foundation of Jiangsu Province [BK20170034]
- Key Research and Development Program of Jiangsu Province [BE2017162]
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We report a computational 3D microscopy technique, termed Fourier ptychographic diffraction tomography (FPDT), that iteratively stitches together numerous variably illuminated, low-resolution images acquired with a low-numerical aperture (NA) objective in 3D Fourier space to create a wide field-of-view (FOV), high-resolution, depth-resolved complex refractive index (RI) image across large volumes. Unlike conventional optical diffraction tomography (ODT) approaches that rely on controlled bright-field illumination, holographic phase measurement, and high-NA objective detection, FPDT employs tomographic RI reconstruction from low-NA intensity-only measurements. In addition, FPDT incorporates high-angle dark-field illuminations beyond the NA of the objective, significantly expanding the accessible object frequency. With FPDT, we present the highest-throughput ODT results with 390 nm lateral resolution and 899 nm axial resolution across a 10 xFOV of 1.77 mm(2) and a depth of focus of similar to 20 mu m. Billion-voxel 3D tomographic imaging results of biological samples establish FPDT as a powerful non-invasive and label-free tool for high-throughput 3D microscopy applications.
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