Journal
NUTRITION RESEARCH
Volume 79, Issue -, Pages 50-59Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.nutres.2020.05.010
Keywords
Ascorbic acid; Phagocytosis; Oxidative burst; Hyperglycemia; Reactive oxygen species
Categories
Funding
- Blackmores Institute
- Royal Golden Jubilee Doctoral Program [PHD/0016/2557, PHD/0045/2553]
- Thailand Science Research and Innovation, Thailand
- Exercise and Sport Sciences Development and Research Group
- Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand
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This study investigated the effect of vitamin C on polymorphonuclear (PMN) cell functions in type 2 diabetes mellitus patients with poor glycemic control. We hypothesized that oral vitamin C treatment improves PMN cell functions. Patients (14) received either a vitamin C (1000 mg/d) or placebo (anhydrous calcium hydrogen phosphate) tablet for 6 weeks and were subjected to a 6-week washout period followed by a 6-week treatment crossover period. Blood samples were collected at pretreatment and posttreatment for PMN cell functions (by flow cytometry) and plasma vitamin C concentration. Phagocytosis was examined by incubating whole blood samples with fluorescein isothiocyanate-labeled Staphylococcus aureus, and oxidative burst was simultaneously evaluated by adding hydroethidine. In comparison with placebo, vitamin C increased both PMN cell phagocytosis (pretreatment placebo, 17.8% +/- 1.6% and vitamin C, 19.0% +/- 3.4%, P = .70; posttreatment: placebo, 16.6% +/- 1.7% and vitamin C, 27.1% +/- 2.9%, P = .005) and oxidative burst (pretreatment placebo, 6.4% +/- 0.8% and vitamin C, 7.1% +/- 1.2%, P = .60; posttreatment placebo, 6.9% +/- 1.3% and vitamin C, 12.1% +/- 1.6%, P = .02). The plasma vitamin C concentration was elevated after vitamin C treatment as compared with that before treatment (P < .001) and was higher than that observed in the placebo treatment group (P < .01). Plasma vitamin C concentration and PMN cell functions were not significantly different before both treatments. We conclude that the 6-week 1000-mg/d vitamin C increased PMN phagocytosis and oxidative burst in type 2 diabetes mellitus patients with poor glycemic control. (C) 2020 The Authors. Published by Elsevier Inc.
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