Journal
NUCLEIC ACIDS RESEARCH
Volume 48, Issue 8, Pages 4161-4178Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa145
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Funding
- Deutsche Forschungsgemeinschaft (DFG) [AI23/9-1]
- Graduate School of Quantitative Biosciences Munich (QBM)
- DFG [CRC 1064/Z03]
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During interphase centromeres often coalesce into a small number of chromocenters, which can be visualized as distinct, DAPI dense nuclear domains. Intact chromocenters play a major role in maintaining genome stability as they stabilize the transcriptionally silent state of repetitive DNA while ensuring centromere function. Despite its biological importance, relatively little is known about the molecular composition of the chromocenter or the processes that mediate chromocenter formation and maintenance. To provide a deeper molecular insight into the composition of the chromocenter and to demonstrate the usefulness of proximity-based biotinylation as a tool to investigate those questions, we performed super resolution microscopy and proximity-based biotinylation experiments of three distinct proteins associated with the chromocenter in Drosophila. Our work revealed an intricate internal architecture of the chromocenter suggesting a complex multilayered structure of this intranuclear domain.
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