4.1 Article

Morphological and molecular characterisation of Distolabrellus veechi (Rhabditida: Mesorhabditidae) from India

Journal

NEMATOLOGY
Volume 22, Issue 4, Pages 438-451

Publisher

BRILL
DOI: 10.1163/15685411-00003315

Keywords

18S rRNA; 28S rRNA; D2-D3; description; Galleria mellonella; ITS rRNA; morphology; phylogenetic analyses; SEM; taxonomy

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Funding

  1. research project `PAIUJA 2019/2020 of the University of Jaen, Spain [EI_RNM02_2019]
  2. Department of Science and Technology (DST), New Delhi, India [SR/WOS-A/LS-1083/2014]

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A redescription of Distolabrellus veechi (Rhabditida: Mesorhabditidae) from agricultural soils in Uttarakhand and Uttar Pradesh, India, is provided. Specimens were extracted from soil using the Galleria soil baiting technique, with two strains of nematodes named JP1 and JP2 being obtained. Morphological and morphometric studies on the species agree well with previous records. Molecular analyses are provided using 18S, 28S and ITS rRNA sequences, the ITS sequences being obtained for the first time in this species. These analyses show that the material examined agrees well with previously studied populations. Phylogenetic analyses showed Distolabrellus to be the sister group of Crustorhabditis and Teratorhabditis. all having the male spicules fused for more than 50% of their length. Illustrations and phylogenetic trees based on 18S, 28S and ITS rRNA sequences are provided.

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